Bt Transgenic Test Strip (CryAb/Ac)

Introduction:

Bt transgenic CryAb / Ac rapid test card application of double antibody sandwich immunochromatography principle, in the surge process, the sample antigen in the first colloidal gold labeled antibody A binding, and then with the detection line specific monoclonal antibody B Combined to form a double antibody sandwich complex. If the antigen content in the sample is greater than the limit, the detection line is red, the result is positive; the other hand, the test line is not color, the result is negative. This product is suitable for leaf tissue and corn, cotton and other crops of the seed detection. The detection of seed, seed, and seed samples must be ground and diluted with buffer or water. In order to obtain the best results, different samples were diluted with different proportions of water.

Sample processing
1, seed
A single seed sample can be ground and extracted in a tube with 10 mmol of phosphate buffer (PB) or distilled water.
Reference to the extraction ratio Weigh the crushed seed sample into the test tube and add the corresponding amount of PB or distilled water. Oscillation after 15-25 seconds. Until the solid material precipitates to the bottom of the test tube, the sample solution can be tested.
The ratio of PB or distilled water to the weight of the seed sample is 5: 1.
2, leaves
A leaf of 1 inch by 1 inch was cut from the leaves and placed in a test tube. Then add 10 drops of PB or distilled water to the tube and crush the tissue. Oscillation 15-25 seconds after standing, until the solid material to the bottom of the test tube, the sample solution can be tested.

3, the extraction ratio

Steps
1, the test tube rewarming to room temperature and then open, remove the test paper to be used immediately after the test tube cover tightly sealed.
2. Always hold the top of the test strip with the test name printed on it. Keep the card in a vertical state and wipe the bottom of the card into the tube of the treated sample solution. The sample solution must not exceed the “Max” line.
3, wait 2-5 minutes to determine the test results.
4, if only one line appears, then wait 10 minutes after the results to judge. 30 minutes after the test results are invalid.

Results Interpretation:

Specifications: 25 / box.
Test method: double antibody sandwich immunochromatography.
Detection limit: 2 ng / ml or 1/200 core.