LH Ovulation Test

Introduction

Human luteinizing hormone (hLH) is a glycoprotein hormone secreted by the anterior pituitary. In view of the characteristic variation of hLH during the menstrual cycle, and sensitive measurement of hLH is an important tool in the diagnosis and management of infertility in females. Approximately 12 – 24 hours after the hLH surge, the wall of the enlarged follicle ruptures at ovulation and the mature ovum is extruded. Detection of the hLH surge can aid in predicting the time of ovulation. The onset of the hLH surge precedes ovulation by approximately 30 hours.

The LH ovulation test is an in-vitro immunochromatographic one step assay designed for qualitative determination of hLH in urine to predict time of ovulation.

The LH ovulation test contains a membrane strip, which is precoated with mouse monoclonal anti-hLH capture antibody on test band region. The mouse monoclonal anti-beta hLH -colloid gold conjugate and urine sample moves along the membrane chromatographically to the test region (T) and forms a visible line as the antibody-antigen-antibody gold particle complex forms. The LH ovulation test device has a letter of T and C as “Test Line” and “Control Line” on the surface of the case. Both the Test Line and Control Line in result window are not visible before applying any samples. The Control Line is used for procedural control. Control line should always appear if the test procedure is performed properly and the test reagents of control line are working. Also, this control band serves as a reference of the color intensity of approximately 35 mIU/ml hLH. When the intensity of the test band is equal or higher than that of control band, the test is positive, indicating the hLH surge is.

 Kit Precautions and Storage Instructions

1) For best results, adhere to instructions provided

2) All specimens should be handled as potentially infectious

3) The test device should be stored at room temperature

4) The test device is sensitive to humidity as well as heat

5) Do not use beyond expiration date

6) Do not use test kit if pouch is damaged or seal is broken

7) Use test device immediately after removing from the pouch

8) The components (test device and assay diluents) in this kit have been quality control tested as a standard batch unit. Do not mix components from different lot numbers.

9) Store kit at room temperature (2 -30 ℃). Do not expose the kit to temperature over 30℃.

10) The stability of the test under above conditions is 24 months from the date of manufacturing.